Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

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10.2478_ffp-2019-0025.pdf (586.28 KB)

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2019-12-30

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Sciendo (De Gruyter)

Abstract

Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

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Acknowledgements: The authors would like to express special thanks to the Indonesia Ministry of Research, Technology and Higher Education for funding this research (718/UN6.3.1/PL/2017). The authors also thank Bogor Botanical Garden for providing with sample materials. The authors also like to thank Novio Ananti Yusril for assisting in the process of taking the sample material and Prof. Wallace A. Cowling from The University of Western Australian and Dr. Annaliese S Mason from Justus Liebig University for the discussions. © 2019 Annisa et al., published by Sciendo. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License - https://creativecommons.org/licenses/by-nc-nd/3.0 . The Version of Scholarly Record of this Article is published in Folia Forestalia Polonica, Series A – Forestry, 2019, Vol. 61 (4), 255–266, available online at: https://sciendo.com/article/10.2478/ffp-2019-0025 . Keywords: bamboo; genetics diversity; RAPD; variation; Asia; Indonesia.

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Annisa, Hafzari, R., Setiawati, T., Irawan, B. & Kusmoro, J. (2019). Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia. Folia Forestalia Polonica, 61(4) 255-266. https://doi.org/10.2478/ffp-2019-0025

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https://hdl.handle.net/20.500.14096/132

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Forest Sciences