Effect of long-term fertilisation on enzyme activities and microbial community composition in the rice rhizosphere

Li, Weitao
Kuzyakov, Yakov
Zheng, Yulong
Liu, Ming
Wu, Meng
Dong, Yuanha
Li, Zhongpei
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Taylor & Francis Group - Informa UK Limited
Mineral fertilisers differ in changing soil properties, and revealing how the rhizosphere and non-rhizosphere respond could provide a robust assessment of fertiliser regimes. Rhizosphere and non-rhizosphere soils were sampled from five fertilisation treatments in a long-term (24 year) experiment. Enzyme activities and total phospholipid fatty acids (PLFA) content in the rhizosphere soil were 85.8% and 51.3% higher than in the non-rhizosphere soil, respectively. Fertilisation increased enzyme activities, especially the N-cycling enzyme β-1,4-N-acetylglucosaminidase in NP fertilised soil (1.5 and 2.5 times for rhizosphere and non rhizosphere soil, respectively). The PLFA composition indicated that fungi cominated in the rhizosphere fertilised with P, whereas bacteria were more common in the non-rhizosphere soil. The PLFA contents and enzyme activities in the rhizosphere of P-fertilised plants were lower than those in the non-rhizosphere soil because P availability was lower in the rhizosphere. The redundancy analysis showed that the microbial community in the rhizosphere soil was different from that of the non-rhizosphere soil, mainly because there were differences in the 15:1ω6c and 16:0. Long-term (24 year) fertilisation strongly increased nutrient contents, and microbial biomass and activity in paddy soil. It is advisable to apply P fertiliser in the root zone to increase fertiliser use efficiency.
The Version of Scholarly Record of this Article is published in Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 2022, available online at: http://www.tandfonline.com/10.1080/09064710.2021.2011394. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Keywords: Long-term fertilisation experiment; rhizosphere properties; enzyme activities; phospholipid fatty acids; Asia; China; sub-tropical; monsoon; nutrient flux. Supplemental data for this article can be accessed at https://doi.org/10.1080/09064710.2021.2011394
Weitao Li, Yakov Kuzyakov, Yulong Zheng, Ming Liu, Meng Wu, Yuanhua Dong & Zhongpei Li (2022) Effect of long-term fertilisation on enzyme activities and microbial community composition in the rice rhizosphere, Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 72:1, 454-462, DOI: 10.1080/09064710.2021.2011394