Fungi isolated from shoots showing ash dieback in the Wolica Nature Reserve in Poland and artificially inoculated seedlings with Hymenoscyphus fraxineus.

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dc.contributor.authorŻółciak, Anna
dc.contributor.authorNowakowska, Justyna Anna
dc.contributor.authorPacia, Artur
dc.contributor.authorKeča, Nenad
dc.contributor.authorOszako, Tomasz
dc.date.accessioned2022-11-30T04:30:05Z
dc.date.available2022-11-30T04:30:05Z
dc.date.issued2019-05-04
dc.description© 2019 Anna Żółciak et al., published by Sciendo. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License - https://creativecommons.org/licenses/by-nc-nd/3.0/legalcode . The Version of Scholarly Record of this Article is published in Folia Forestalia Polonica, Series A – Forestry, 2019, Vol. 61 (1), 42–50, available online at: https://sciendo.com/article/10.2478/ffp-2019-0004 . Keywords: bark necrosis; ash dieback; Fraxinus excelsior; Hymenoscyphus fraxineus; ITS1-F; Europe; Poland.
dc.description.abstractAsh dieback caused by an alien, invasive fungus Hymenoscyphus fraxineus is a serious disease of European ash species in many parts in Europe. In Poland, the disease was recorded in the beginning of the 1990s. This study was performed in 2016–2017 with the aim to identify fungi isolated from ash shoots showing dieback symptoms in the Wolica Nature Reserve in Poland, as well as from shoots of two years-old ash seedlings inoculated with H. fraxineus in the greenhouse. The most frequently isolated fungi from shoots of common ash (associated with the pathogenic fungus H. frax ineus) were identified on the basis of sequencing of the internal transcribed spacer region (ITS1) of fungal rDNA. In total, 19 fungal taxa were identified for ash shoots as follows: H. fraxineus, Fusarium avenaceum, Alternaria spp., Phomopsis oblonga, Diplodia mutila and other Phomopsis spp. The pathogen H. fraxineus was not found for all the shoots samples; one year after inoculation the aforementioned fungi and other species as: Alternaria alternata, Bionectria ochroleuca, Epicoccum nigrum, F. acuminatum, F. avenaceum, and Paraphaesphaeria neglecta were identified in inoculation point, as well as H. fraxineus. The same quantitative and qualitative changes of organisms were observed in the case of artificially colonised ash seedlings in the greenhouse, as well as in the shoots of adult ash trees in the forest.
dc.description.sponsorshipAuthors thank Małgorzata Gorzkowska for the ITS1 rDNA analysis, and IBL funds supporting 3 months stay of Prof. Nenad Keča in the Department of Forest Protection in Sękocin Stary. The present research was supported by the Ministry of Science and Higher Education of Poland as the part of project no. 240 325.
dc.identifier.citationŻółciak, A., Nowakowska, J., Pacia, A., Keča, N. & Oszako, T. (2019). Fungi isolated from shoots showing ash dieback in the Wolica Nature Reserve in Poland and artificially inoculated seedlings with Hymenoscyphus fraxineus. Folia Forestalia Polonica, 61(1) 42-50. https://doi.org/10.2478/ffp-2019-0004
dc.identifier.otherhttps://doi.org/10.2478/ffp-2019-0004
dc.identifier.urihttps://hdl.handle.net/20.500.14096/110
dc.language.isoen
dc.publisherSciendo (De Gruyter)
dc.titleFungi isolated from shoots showing ash dieback in the Wolica Nature Reserve in Poland and artificially inoculated seedlings with Hymenoscyphus fraxineus.
dc.typeArticle

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